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sterile polystyrene inoculating loop os101  (Corning Life Sciences)

 
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    Corning Life Sciences sterile polystyrene inoculating loop os101
    Sterile Polystyrene Inoculating Loop Os101, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sterile+polystyrene+inoculating+loop/sterile+polystyrene+inoculating+loop+os101/pmc07309966-12-21-5
    Average 90 stars, based on 1 article reviews
    sterile polystyrene inoculating loop os101 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sterility:

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: In addition, during the genomic library preparation, do not allow the beads to fully dry by shortening the bead drying time to 1–2 min. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Adenine, Powder Acros Organics 147441000 Use at 75 mg/L to make liquid and solid rich media (YEA) Bacteriology Petri Dish Corning, Falcon C351029 100*15mm, use to grow strains to single colonies on solid rich media D-Glucose Anhydrous, Powder Fisher Chemical D16–1 Use at 30 g/L to make liquid and solid rich media (YEA) Difco Agar, Granuated Becton, Dickinson and Co. 214530 Use at 20 g/L to make solid rich media (YEA) DNA extraction buffer 2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-Cl (pH 8.0), 1mM Na 2 -EDTA Focused-ultrasonicator Covaris Inc. S220 Alternatively, use QSonica Q800R sonicator/DNA and chromatin shearing system Gen5 Data Collection and Analysis Software Biotek, Inc. GEN5SECURE Or equivalent, must be compatible with the micro-plate reader, use to export data readings from the micro-plate reader Hydrochloric Acid 1N, Liquid Fisher Chemical SA48–4 Use to adjust pH to 5.5 in liquid and solid rich media Liquid Rich Media (liquid YEA) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, pH adjusted to 5.5 with 1 M HCl Microplate Reader, Synergy H1 Hybrid Multi-Mode Reader Biotek, Inc. BTH1MG Or equivalent, must read visible light at 600nm wavelength range Rich Media agar plates (YEA plates) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, 20 g/L Agar, pH adjusted to 5.5 with 1 M HCl. .. RNase A/T1 mix Thermo Fisher Scientific EN0551 Use according to manufacturer recommendation Sterile Polystyrene Inoculating Loop Corning, Inc. OS101 Or equivalent, use to transfer colonies from agar plates to 96- well plate Sterile workspace and burners Tissue Culture Plate, 96-well Optical Flat Bottom with Low Evaporation Lid Corning, Falcon C353072 Or equivalent, must have optical flat bottom for micro-plate ready TruSeq DNA PCR-Free LT/HT Library Prep Kit Illumina, Inc. 20015962 Use to prepare the whole-genome sequencing library Yeast Extract, Powder Fisher Chemical BP1422–500 Use at 5 g/L to make liquid and solid rich media (YEA) Open in a separate window Materials For shearing parameters during the library preparation, use a focused sonicator (see ) and set the duty factor to 20%, peak power to 175 W, with 200 cycles per burst, and frequency sweeping mode at 5.5oC to 6 °C for 45 s. Alternatively, use a DNA and chomatin shearing system (see ) with the following settings: 50% amplitude at 4 °C with pulse mode, 15 s on and 15 s off for 10 min, with a total processing time of 20 min. ..

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: RNase A/T1 mix , Thermo Fisher Scientific , EN0551 , Use according to manufacturer recommendation. .. Sterile Polystyrene Inoculating Loop , Corning, Inc. , OS101 , Or equivalent, use to transfer colonies from agar plates to 96- well plate. .. Sterile workspace and burners , , , .

    Evaporation:

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: In addition, during the genomic library preparation, do not allow the beads to fully dry by shortening the bead drying time to 1–2 min. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Adenine, Powder Acros Organics 147441000 Use at 75 mg/L to make liquid and solid rich media (YEA) Bacteriology Petri Dish Corning, Falcon C351029 100*15mm, use to grow strains to single colonies on solid rich media D-Glucose Anhydrous, Powder Fisher Chemical D16–1 Use at 30 g/L to make liquid and solid rich media (YEA) Difco Agar, Granuated Becton, Dickinson and Co. 214530 Use at 20 g/L to make solid rich media (YEA) DNA extraction buffer 2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-Cl (pH 8.0), 1mM Na 2 -EDTA Focused-ultrasonicator Covaris Inc. S220 Alternatively, use QSonica Q800R sonicator/DNA and chromatin shearing system Gen5 Data Collection and Analysis Software Biotek, Inc. GEN5SECURE Or equivalent, must be compatible with the micro-plate reader, use to export data readings from the micro-plate reader Hydrochloric Acid 1N, Liquid Fisher Chemical SA48–4 Use to adjust pH to 5.5 in liquid and solid rich media Liquid Rich Media (liquid YEA) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, pH adjusted to 5.5 with 1 M HCl Microplate Reader, Synergy H1 Hybrid Multi-Mode Reader Biotek, Inc. BTH1MG Or equivalent, must read visible light at 600nm wavelength range Rich Media agar plates (YEA plates) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, 20 g/L Agar, pH adjusted to 5.5 with 1 M HCl. .. RNase A/T1 mix Thermo Fisher Scientific EN0551 Use according to manufacturer recommendation Sterile Polystyrene Inoculating Loop Corning, Inc. OS101 Or equivalent, use to transfer colonies from agar plates to 96- well plate Sterile workspace and burners Tissue Culture Plate, 96-well Optical Flat Bottom with Low Evaporation Lid Corning, Falcon C353072 Or equivalent, must have optical flat bottom for micro-plate ready TruSeq DNA PCR-Free LT/HT Library Prep Kit Illumina, Inc. 20015962 Use to prepare the whole-genome sequencing library Yeast Extract, Powder Fisher Chemical BP1422–500 Use at 5 g/L to make liquid and solid rich media (YEA) Open in a separate window Materials For shearing parameters during the library preparation, use a focused sonicator (see ) and set the duty factor to 20%, peak power to 175 W, with 200 cycles per burst, and frequency sweeping mode at 5.5oC to 6 °C for 45 s. Alternatively, use a DNA and chomatin shearing system (see ) with the following settings: 50% amplitude at 4 °C with pulse mode, 15 s on and 15 s off for 10 min, with a total processing time of 20 min. ..

    Polymerase Chain Reaction:

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: In addition, during the genomic library preparation, do not allow the beads to fully dry by shortening the bead drying time to 1–2 min. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Adenine, Powder Acros Organics 147441000 Use at 75 mg/L to make liquid and solid rich media (YEA) Bacteriology Petri Dish Corning, Falcon C351029 100*15mm, use to grow strains to single colonies on solid rich media D-Glucose Anhydrous, Powder Fisher Chemical D16–1 Use at 30 g/L to make liquid and solid rich media (YEA) Difco Agar, Granuated Becton, Dickinson and Co. 214530 Use at 20 g/L to make solid rich media (YEA) DNA extraction buffer 2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-Cl (pH 8.0), 1mM Na 2 -EDTA Focused-ultrasonicator Covaris Inc. S220 Alternatively, use QSonica Q800R sonicator/DNA and chromatin shearing system Gen5 Data Collection and Analysis Software Biotek, Inc. GEN5SECURE Or equivalent, must be compatible with the micro-plate reader, use to export data readings from the micro-plate reader Hydrochloric Acid 1N, Liquid Fisher Chemical SA48–4 Use to adjust pH to 5.5 in liquid and solid rich media Liquid Rich Media (liquid YEA) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, pH adjusted to 5.5 with 1 M HCl Microplate Reader, Synergy H1 Hybrid Multi-Mode Reader Biotek, Inc. BTH1MG Or equivalent, must read visible light at 600nm wavelength range Rich Media agar plates (YEA plates) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, 20 g/L Agar, pH adjusted to 5.5 with 1 M HCl. .. RNase A/T1 mix Thermo Fisher Scientific EN0551 Use according to manufacturer recommendation Sterile Polystyrene Inoculating Loop Corning, Inc. OS101 Or equivalent, use to transfer colonies from agar plates to 96- well plate Sterile workspace and burners Tissue Culture Plate, 96-well Optical Flat Bottom with Low Evaporation Lid Corning, Falcon C353072 Or equivalent, must have optical flat bottom for micro-plate ready TruSeq DNA PCR-Free LT/HT Library Prep Kit Illumina, Inc. 20015962 Use to prepare the whole-genome sequencing library Yeast Extract, Powder Fisher Chemical BP1422–500 Use at 5 g/L to make liquid and solid rich media (YEA) Open in a separate window Materials For shearing parameters during the library preparation, use a focused sonicator (see ) and set the duty factor to 20%, peak power to 175 W, with 200 cycles per burst, and frequency sweeping mode at 5.5oC to 6 °C for 45 s. Alternatively, use a DNA and chomatin shearing system (see ) with the following settings: 50% amplitude at 4 °C with pulse mode, 15 s on and 15 s off for 10 min, with a total processing time of 20 min. ..

    Sequencing:

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: In addition, during the genomic library preparation, do not allow the beads to fully dry by shortening the bead drying time to 1–2 min. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Adenine, Powder Acros Organics 147441000 Use at 75 mg/L to make liquid and solid rich media (YEA) Bacteriology Petri Dish Corning, Falcon C351029 100*15mm, use to grow strains to single colonies on solid rich media D-Glucose Anhydrous, Powder Fisher Chemical D16–1 Use at 30 g/L to make liquid and solid rich media (YEA) Difco Agar, Granuated Becton, Dickinson and Co. 214530 Use at 20 g/L to make solid rich media (YEA) DNA extraction buffer 2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-Cl (pH 8.0), 1mM Na 2 -EDTA Focused-ultrasonicator Covaris Inc. S220 Alternatively, use QSonica Q800R sonicator/DNA and chromatin shearing system Gen5 Data Collection and Analysis Software Biotek, Inc. GEN5SECURE Or equivalent, must be compatible with the micro-plate reader, use to export data readings from the micro-plate reader Hydrochloric Acid 1N, Liquid Fisher Chemical SA48–4 Use to adjust pH to 5.5 in liquid and solid rich media Liquid Rich Media (liquid YEA) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, pH adjusted to 5.5 with 1 M HCl Microplate Reader, Synergy H1 Hybrid Multi-Mode Reader Biotek, Inc. BTH1MG Or equivalent, must read visible light at 600nm wavelength range Rich Media agar plates (YEA plates) 30 g/L D-Glucose, 5 g/L Yeast Extract, 75 mg/L Adenine, 20 g/L Agar, pH adjusted to 5.5 with 1 M HCl. .. RNase A/T1 mix Thermo Fisher Scientific EN0551 Use according to manufacturer recommendation Sterile Polystyrene Inoculating Loop Corning, Inc. OS101 Or equivalent, use to transfer colonies from agar plates to 96- well plate Sterile workspace and burners Tissue Culture Plate, 96-well Optical Flat Bottom with Low Evaporation Lid Corning, Falcon C353072 Or equivalent, must have optical flat bottom for micro-plate ready TruSeq DNA PCR-Free LT/HT Library Prep Kit Illumina, Inc. 20015962 Use to prepare the whole-genome sequencing library Yeast Extract, Powder Fisher Chemical BP1422–500 Use at 5 g/L to make liquid and solid rich media (YEA) Open in a separate window Materials For shearing parameters during the library preparation, use a focused sonicator (see ) and set the duty factor to 20%, peak power to 175 W, with 200 cycles per burst, and frequency sweeping mode at 5.5oC to 6 °C for 45 s. Alternatively, use a DNA and chomatin shearing system (see ) with the following settings: 50% amplitude at 4 °C with pulse mode, 15 s on and 15 s off for 10 min, with a total processing time of 20 min. ..



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    Image Search Results


    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

    doi: 10.3791/59133

    Figure Lengend Snippet: Materials

    Article Snippet: RNase A/T1 mix Thermo Fisher Scientific EN0551 Use according to manufacturer recommendation Sterile Polystyrene Inoculating Loop Corning, Inc. OS101 Or equivalent, use to transfer colonies from agar plates to 96- well plate Sterile workspace and burners Tissue Culture Plate, 96-well Optical Flat Bottom with Low Evaporation Lid Corning, Falcon C353072 Or equivalent, must have optical flat bottom for micro-plate ready TruSeq DNA PCR-Free LT/HT Library Prep Kit Illumina, Inc. 20015962 Use to prepare the whole-genome sequencing library Yeast Extract, Powder Fisher Chemical BP1422–500 Use at 5 g/L to make liquid and solid rich media (YEA) Open in a separate window Materials For shearing parameters during the library preparation, use a focused sonicator (see ) and set the duty factor to 20%, peak power to 175 W, with 200 cycles per burst, and frequency sweeping mode at 5.5oC to 6 °C for 45 s. Alternatively, use a DNA and chomatin shearing system (see ) with the following settings: 50% amplitude at 4 °C with pulse mode, 15 s on and 15 s off for 10 min, with a total processing time of 20 min.

    Techniques: DNA Extraction, Software, Evaporation, Sequencing